American Society of Hematology

Fig. 1.

$Fig. 1. Experimental design. Rhesus macaques underwent mobilization with G-CSF/SCF followed by apheresis of 2.5 times their blood volume. The apheresis product was enriched for primitive progenitors by CD34 selection and split into two equal fractions for a 96-hour transduction with either of two retroviral vectors: G1 (G1Na) or LN (LNL6). One aliquot was frozen at the end of transduction, whereas the other was returned to the original culture conditions without further exposure to retrovirus for a total of 10 to 14 days before freezing. Culture condition A: IL-3, IL-6, SCF; culture condition B: IL-3, IL-6, SCF, Flt-3 ligand; culture condition C: IL-3, IL-6, SCF, Flt-3 ligand, and an autologous stromal monolayer. After 650 cGy TBI on 2 consecutive days, both the transduced (nonexpanded) and the transduced and expanded aliquots were thawed and simultaneously reinfused.$

Experimental design. Rhesus macaques underwent mobilization with G-CSF/SCF followed by apheresis of 2.5 times their blood volume. The apheresis product was enriched for primitive progenitors by CD34 selection and split into two equal fractions for a 96-hour transduction with either of two retroviral vectors: G1 (G1Na) or LN (LNL6). One aliquot was frozen at the end of transduction, whereas the other was returned to the original culture conditions without further exposure to retrovirus for a total of 10 to 14 days before freezing. Culture condition A: IL-3, IL-6, SCF; culture condition B: IL-3, IL-6, SCF, Flt-3 ligand; culture condition C: IL-3, IL-6, SCF, Flt-3 ligand, and an autologous stromal monolayer. After 650 cGy TBI on 2 consecutive days, both the transduced (nonexpanded) and the transduced and expanded aliquots were thawed and simultaneously reinfused.

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