Interaction between GFP-pICln and GST/P4.1 proteins. SiHa cells were electroporated with a GFP-pICln cDNA. The whole cell lysates prepared from transfected cells (lane 1) were incubated with GST/P80 (lane 3), GST/P30 (lane 4), or GST/P10+24 (lane 5) fusion proteins previously coupled to glutathione sepharose beads. The bound complexes were electrophoresed, transferred to the membrane, and analyzed by a polyclonal antibody against GFP. Lane 2 is a negative control. The cell lysate was prepared from nontransfected SiHa cells and analyzed by anti-GFP antibody.