Protein 4.1 interacts with pICln protein in the two-hybrid screen. Interaction between various portions of protein 4.1 and pICln using a colony-lift assay (A) or a liquid assay for β-galactosidase activity (B). “−” in (A) is a negative control transformation by both a pLAM5′-1 plasmid encoding a GAL4 DNA-BD/human lamin C hybrid and a GAL4-AD-pICln DNA.