Detection of the ZNF198/FGFR1 fusion product in patient SR13 and somatic cell hybrid RBF1.6First-strand cDNA synthesis was generated from 1 μg of total RNA, using random hexamers as primers according to standard protocols. Oligonucleotides were designed to sites in the ZNF198 and FGFR1 cDNA flanking the translocation breakpoints: ZNF198F (5′-ccctgtgcctgtgtatatcccag-3′), ZNF198R (5′-tgcaggaatcttctcactgc-3′), FGFRX7F (5′-gatcatcgtctacaagatg-3′), and FGFR1979R (5′-gtgatggccgaaccagaagaac-3′). Lanes 1 and 2, ZNF198/FGFR1 fusion products detected in RBF1 and SR, respectively, by primers ZNF198F/FGF1979R. Lanes 4 and 5, potential FGFR1/ZNF198 fusion products detected by primers FGFX7F/ZNF198R in RBF1 and SR. Lanes 7 and 8, expression of the normal ZNF198 mRNA in RBF1 and SR, detected using primers ZNF198F/ZNF198R. Lanes 10 and 11, FGFR1 expression in RBF1 and SR, respectively, detected with primers FGFRX7F/FGF1979R. Lanes 3, 6, 9, and 12, negative controls.
