CD95 and CD95L expression following resveratrol treatment of HL60 cells. (A) HL60 cells were treated with 32 μmol/L resveratrol, and surface expression of CD95 and CD95L was analyzed by flow cytometry at 6 hours, 12 hours, and 24 hours as described in Materials and Methods. The shaded histograms are for mouse IgG₁κ used as an isotype control, the dotted lines represent nontreated HL60 cells, and the solid lines indicate resveratrol-treated HL60 cells. At least 10,000 events were counted, and data shown are representative of at least three separate experiments. (B) RT-PCR amplification of CD95L and β-actin mRNA following treatment of HL60 cells with 32 μmol/L resveratrol for 0, 4, 8, and 20 hours.