Resveratrol induces DNA fragmentation and loss of membrane phospholipid asymmetry. (A) Flow cytometry analysis of DNA cleavage in resveratrol-treated tumor cells. HL60 cells were treated with 32 μmol/L resveratrol for 18 hours, immediately fixed in ethanol, and stained with PI for DNA content analysis as described in Materials and Methods. Sub-G1 population indicates subdiploid DNA content indicative of apoptotic DNA fragmentation. Data shown are representative of at least three independent experiments. (B) Externalization of membrane PS induced by resveratrol in HL60 cells. 1 × 106 cells were treated with resveratrol for 18 hours, and PS translocation was assessed by staining tumor cells with Annexin V-FITC (1 μg/mL) conjugate. A total of 10,000 events were analyzed by flow cytometry.
