Fig. 5.
Fig. 5. Production of TNF-α from different populations of mast cells stimulated with either MBP or poly-L-arginine. BMCMC in SCF, BMCMC in CM, or Cl.MC/C57.1 mast cells were stimulated with either MBP (10−6 mol/L) (A) or poly-L-arginine (10-5mol/L) (B) for 30 minutes. Supernatants were then collected and analyzed for TNF-α by ELISA. Cell viability was greater than 90% as determined by Trypan blue exclusion. The data are expressed as mean ± SD (n = 3). ***P < .05 by the two-tailed paired Student's t-test.

Production of TNF-α from different populations of mast cells stimulated with either MBP or poly-L-arginine. BMCMC in SCF, BMCMC in CM, or Cl.MC/C57.1 mast cells were stimulated with either MBP (10−6 mol/L) (A) or poly-L-arginine (10-5mol/L) (B) for 30 minutes. Supernatants were then collected and analyzed for TNF-α by ELISA. Cell viability was greater than 90% as determined by Trypan blue exclusion. The data are expressed as mean ± SD (n = 3). ***P < .05 by the two-tailed paired Student's t-test.

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