Amplification of BM stromal TPO mRNA by TaqMan RT-PCR method in NS and in patients with ITP before and after treatment, AA, and ET. Two hundred nanograms of total cellular RNA extracted from stromal cells of NS (n = 6) and patients with ITP before (n = 5) and after (n = 6) treatment with steroid, AA (n = 6), and ET (n = 2) was subjected to TaqMan RT-PCR method. Threshold Rn value and CT were obtained as described in Materials and Methods. In (A) and (B), curves represent amplification plots of GAPDH mRNA and TPO mRNA, respectively. In (C), magnifying view of rectangular area of (B) which included minimum and maximum CT value for each disease group was shown to illustrate the differences more apparently.