American Society of Hematology

Fig. 3.

$Fig. 3. The effect of apoptosis on PKCα and δ distribution in cytosolic and particulate fractions. TF-1 cells were treated with γ radiation (5 Gy), incubated in either the presence or absence of GM-CSF (100 U/mL) in complete RPMI supplemented with 20% FBS, and harvested at 5 minutes and 1 and 6 hours postirradiation. Nonnuclear cytosolic and particulate fractions were analyzed for PKCδ isoform expression by immunoblotting. This is an autoradiograph of a representative blot of three showing PKC expression.$

The effect of apoptosis on PKCα and δ distribution in cytosolic and particulate fractions. TF-1 cells were treated with γ radiation (5 Gy), incubated in either the presence or absence of GM-CSF (100 U/mL) in complete RPMI supplemented with 20% FBS, and harvested at 5 minutes and 1 and 6 hours postirradiation. Nonnuclear cytosolic and particulate fractions were analyzed for PKCδ isoform expression by immunoblotting. This is an autoradiograph of a representative blot of three showing PKC expression.

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