Time-dependent effect of vWF on fVIII inactivation by BO2C11. rfVIII alone (solid symbols) or rfVIII complexed with purified vWF (open symbols) was mixed with BO2C11 at either one of two concentrations (see below). The mixture was then incubated at 37°C for various periods of time, from 2 to 60 minutes, after which the residual fVIII activity was evaluated in a chromogenic assay. Results are expressed as the percentage of the activity observed in the absence of antibody. All values are given as final concentrations in the assay system. (•) rfVIII at 60 ng/mL (0.22 nmol/L); (○) rfVIII with vWF at 3 μg/mL (12 nmol/L); (▴) rfVIII + BO2C11 at 85 ng/mL (0.57 nmol/L); (▵) rfVIII-vWF + BO2C11 at 85 ng/mL (0.57 nmol/L); (▪) rfVIII + BO2C11 at 85 μg/mL (570 nmol/L); and (□) rfVIII-vWF + BO2C11 at 85 μg/mL (570 nmol/L).