Fig. 7.
![Fig. 7. Antiproliferative effects of doxorubicin, prodrug N-[4-doxorubicin-N-carbonyl(-oxymethyl) phenyl] O-β-glucuronyl carbamate (DOX-GA3), and prodrug combined with scFv-CD20–β-glucuronidase. Cells were incubated with fusion protein, blocking antibody, and fusion protein, or PBS. Cells were then seeded in 96-wells plates and exposed to drug or prodrug for 24 hours. After 72 hours, cell growth was measured with a protein dye, Sulforhodamine B.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/92/1/10.1182_blood.v92.1.184.413k26_184_190/5/blod41326007x.jpeg?Expires=1722429409&Signature=JNGqJtDvZNpP2N1xZwYr9dFyRgBJH5ccN9MQYWK3fGqTqxEDQdKGzLAyk1oVlPDpty5R0~sJqC8~z5eiMewjnD4BwA2bE8i48tU6gu4Teq3poudgR8VsubgVMZ4P3LGnv~razOQ4hRr0MTdtQPZZIgIa8C5TyRe4DdlbQQO1G1ws6yd1hn2j5dXqkttyXNnvF2Wj-CiRClR56cQZp8p9zwZipNv8IclLPh~6genhibVyBWy6Ti1KAuo9G8vcsAKaqmqIocBRmlHnACeFv2ygWJAwBHQeVtlnfXtk6NKJhwsItCcAP6d-U2oub4cMZHZZ0hkbI7n1esfnXINWaUY4~Q__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Antiproliferative effects of doxorubicin, prodrug N-[4-doxorubicin-N-carbonyl(-oxymethyl) phenyl] O-β-glucuronyl carbamate (DOX-GA3), and prodrug combined with scFv-CD20–β-glucuronidase. Cells were incubated with fusion protein, blocking antibody, and fusion protein, or PBS. Cells were then seeded in 96-wells plates and exposed to drug or prodrug for 24 hours. After 72 hours, cell growth was measured with a protein dye, Sulforhodamine B.
