Fig. 6.
![Fig. 6. Hydrolysis of the prodrug N-[4-doxorubicin-N-carbonyl (-oxymethyl) phenyl] O-β-glucuronyl carbamate (100 μmol/L, closed symbols) to the cytotoxic drug doxorubicin (open symbols) by human β-glucuronidase (▵ and ▴, 1 μg/mL) or scFv-CD20–β-glucuronidase (○ and •, 1 μg/mL) as determined by reversed-phase HPLC and fluorescence detection.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/92/1/10.1182_blood.v92.1.184.413k26_184_190/5/blod41326006x.jpeg?Expires=1722430545&Signature=3u9prhZgY6UKHA9zk3tA4Oi56b01KYhkHUtYwh69jY7mLAqRapmhpl~CA0SiLq917bpWmKtr~5oK2fniClVOWmvFwESMNOsq4LEdjP1VKR2szZXN40X6~l2cqzEd1Ye1cBvG9YZA9EKJPHzcULY-JPpoxk~o8pD1YIn2WrKfvWFDm2AjFwVgLODdZSIVxrsoQ~ZwIYeJ5nCEw0MzrMiYzoJVG8736mhm8Ajsl3N0Cap-g2LgZM0H9cxweHqHi8KzqWJnPBQC-yB9TWVRyRZrRAUrziObh0HYy-Bn~seoNFaTGDVbdTjSQFpFIPXrfDyvk43Enn4ub0eQ26NCZi5q9w__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Hydrolysis of the prodrug N-[4-doxorubicin-N-carbonyl (-oxymethyl) phenyl] O-β-glucuronyl carbamate (100 μmol/L, closed symbols) to the cytotoxic drug doxorubicin (open symbols) by human β-glucuronidase (▵ and ▴, 1 μg/mL) or scFv-CD20–β-glucuronidase (○ and •, 1 μg/mL) as determined by reversed-phase HPLC and fluorescence detection.
