Analyses of UCC stimulated for 0 to 24 days with 0 to 10 ng/mL of IL-5. Cells from different donors at days 0, 6, 12, 18, and 24 were analyzed, and the mean for each separate point was determined. Each point represents the mean from at least two separate donors. (a) The percentage of immunofluorescent cells positive for proMBP determined by staining with anti-proMBP J163-15E10. (b) The percentage of cells positive for MBP determined by staining with rabbit #14 anti-MBP. (c) The percentage of viable cells determined by trypan blue uptake. (d) The absolute number of cells ×10⁶/mL.