Fig. 6.
![Fig. 6. Displacement curves of125I-[Ac-His-1]-PACAP(1-27) to tissue sections of a human thymus (A) and a human lymph node (B). Tissue sections are incubated with 15,000 cpm/100 μL radioligand and increasing concentrations of PACAP(1-27) (▴) and VIP (▪), or somatostatin (SS-14) (•) at a concentration of 100 nmol/L. Nonspecific binding was subtracted from all values. In all cases, complete displacement of the ligand is achieved by VIP and PACAP. SS-14 was inactive in the nanomolar range.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/92/1/10.1182_blood.v92.1.191.413k12_191_197/5/blod41312006x.jpeg?Expires=1722713282&Signature=KOim0a1cYHJ3sfqaMgOYLDLJP0VBGO-O6QpacA68fMkf9akxpUNcrFYb2OS~NKP35JnbD6ext~INOfXLyewqw42mTWKbnw7fvAizYTa2n3e0YamaJJW9IG-dCcQFxn43bPhLrY0oLQCGvJ1-y9Czm~EVvexsX80yx-lhaEErNCavuTb~Cl9Gyg8rYal9mz~qHMP7Dk7R5gB4IpvvMwDLtzqK1LtPBy8Nf-58QU~U-1lyKgpKiHtwSh9E30Zt-WxYQTBoEuFyRgIUl7ICRhjZGymHK2Cz5EgYn1LMDw7fE8KpRXzoMJYnV8ZpnsdYpM3HiyY4LZ5CRmPfStwhnlBiHw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Displacement curves of125I-[Ac-His-1]-PACAP(1-27) to tissue sections of a human thymus (A) and a human lymph node (B). Tissue sections are incubated with 15,000 cpm/100 μL radioligand and increasing concentrations of PACAP(1-27) (▴) and VIP (▪), or somatostatin (SS-14) (•) at a concentration of 100 nmol/L. Nonspecific binding was subtracted from all values. In all cases, complete displacement of the ligand is achieved by VIP and PACAP. SS-14 was inactive in the nanomolar range.
