Multiplex RT-PCR and split-out analysis. (A) The two cell lines MV-4-11 and Mono-Mac-6 and patient no. 243 were positive for a translocation in multiplex reaction R5 (see Table 3). (B) To determine the translocation, a split-out PCR analysis was performed using the individual primer sets R5A, R5B, R5C, and R5D. M, DNA molecular weight marker VI (Boehringer).