Western blot analysis of TM and TF in leukemic cell lines treated with 1,25(OH)₂D₃. Cell lysates were subjected to immunoblotting analysis using a monoclonal anti-TM antibody (A), and a monoclonal anti-TF antibody (B). Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) was performed under nonreducing conditions. All cell lines were incubated either with (+) or without (−) 0.1 μmol/L 1,25(OH)₂D₃for 24 hours. Molecular-weight markers are shown along the left margin. Soluble recombinant TM (A, lane 5) and placenta TF (B, lane 3) were used as controls.