Fig. 2.
(A) Characterization of adhesion molecules expressed by DC. After 5 days in vitro with GM-CSF and IL-13, DC were cultured for 2 additional days in the presence (□) or absence (▪) of IL-13. Cells were labeled with the designed MoAb and then with FITC-labeled goat anti-mouse Ig. (B) Mixed leukocyte reaction (MLR). Responder cells were allogeneic T lymphocytes depleted of autologous monocytes and B lymphocytes; they were plated at 1 × 105cells/well. DC cultured for last 2 days with (○) or without (•) of IL-13 were mixed at the indicated concentrations. 3H-Tdr was added in the last 18 hours of a 5-day experiment.
