Fig. 2.
Fig. 2. Investigation into products detected when performing nested PCR-SSP typing with primer mix 118. Seven DNA samples (A to G) were subject to nested PCR-SSP typing and the resultant amplicons were purified and sequenced using 33P cycle sequencing. The resultant amplicon sequences were aligned to known HLA-DRB sequences31 and compared with HLA DRB1*0301 (redundancies are identified using the International Union of Biochemistry Group Codes where K = G/T, M = A/C, R = A/G, S = C/G, V = A/C/G, W = A/T, Y = C/T; * indicates base unknown).

Investigation into products detected when performing nested PCR-SSP typing with primer mix 118. Seven DNA samples (A to G) were subject to nested PCR-SSP typing and the resultant amplicons were purified and sequenced using 33P cycle sequencing. The resultant amplicon sequences were aligned to known HLA-DRB sequences31 and compared with HLA DRB1*0301 (redundancies are identified using the International Union of Biochemistry Group Codes where K = G/T, M = A/C, R = A/G, S = C/G, V = A/C/G, W = A/T, Y = C/T; * indicates base unknown).

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