Fig. 6.
(A) Anti-fas and dexamethasone activate and IL-6 inhibits jun kinase in 8226 cells. 8226 MM cells incubated in media (control) or dexamethasone alone (10−6 mol/L), IL-6 (1,000 U/mL) alone, dexamethasone + IL-6 (IL-6 present for 60 minutes followed by dexamethasone for 10 minutes, anti-fas alone (0.5 μg/mL for 10 minutes), or anti-fas + IL-6 where IL-6 is present for 10, 30, or 60 minutes before addition of anti-fas. Whole-cell lysates were then tested for enzymatic activity against GST-jun in an in vitro kinase assay. (B) Coomassie blue staining of gel shown in (A) to confirm equal protein loading.
