American Society of Hematology

Fig. 5.

$Fig. 5. Distribution of b5R enzyme activity between pellet (□) and supernatant (▪) fractions obtained from the PNS of cells transfected with pCB6 (Vector), M-cDNA (M), S-cDNA (S), or rat b5R S-cDNA (rat S). Enzyme activity in the fractions was assessed by the NADH-MetHb.FeCN reductase assay in the presence of detergent. Specific enzyme activities in the PNS fractions (nmol substrate reduced.min−1. mg protein) were: Vector, 49.9; M, 119; S, 55.2; rat S, 57.$

Distribution of b5R enzyme activity between pellet (□) and supernatant (▪) fractions obtained from the PNS of cells transfected with pCB6 (Vector), M-cDNA (M), S-cDNA (S), or rat b5R S-cDNA (rat S). Enzyme activity in the fractions was assessed by the NADH-MetHb.FeCN reductase assay in the presence of detergent. Specific enzyme activities in the PNS fractions (nmol substrate reduced^.min^−1. mg protein) were: Vector, 49.9; M, 119; S, 55.2; rat S, 57.

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