Fig. 4.
![Fig. 4. Turnover of band 3 in the microsomal and plasma membrane fractions of early and late erythroblasts. (A) Microsomes and plasma membranes were isolated from erythroblasts labeled with [35S]methionine for 30 minutes and chased for different time periods (0 to 120 minutes). Band 3 was immunoprecipitated using anti–band 3 antibodies. The immunoprecipitates were analyzed by SDS-PAGE and the gels were processed for fluorography. B3: Band 3. (B) The autoradiograms shown in (A) were scanned and the area under each peak integrated.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/92/1/10.1182_blood.v92.1.329.413k20_329_338/5/blod41320004w.jpeg?Expires=1724239694&Signature=KTD-vC3kEPVcj5yRPlraX-ayL0p8CVdgch9Up61R-szo0gnhIjQGvVKIL6V5Qr8D8LizM680-nZwzBg4RhPtEMBo9tt2WR3DwuBEjeTcRq11ufxiVlQZXVPiuvcytcy26anTpxbum7YvHB-ybsap5mK~XLG2478vxCOwSOTKMLruONpuAcu06d-txUVG4JlCQF2Crj0pf2g4Z3DbGsPqbwEVNFxlOBPalhb-FIB6IICBe5EOE1J0sICZWh9vyTNAKXIJ2sfhwom~U9Rq9Z4ea46fPQHYVSZKJ5tMcShUAZSgRtegpZ0h9GSIPN4~R2wcSp9ylNcj3CBgpuqlWQkNjA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Turnover of band 3 in the microsomal and plasma membrane fractions of early and late erythroblasts. (A) Microsomes and plasma membranes were isolated from erythroblasts labeled with [35S]methionine for 30 minutes and chased for different time periods (0 to 120 minutes). Band 3 was immunoprecipitated using anti–band 3 antibodies. The immunoprecipitates were analyzed by SDS-PAGE and the gels were processed for fluorography. B3: Band 3. (B) The autoradiograms shown in (A) were scanned and the area under each peak integrated.
