Fig. 4.
![Fig. 4. Stx1 dose response of NF-κB nuclear translocation. (A) Differentiated THP-1 cells or (B) human PBMn were incubated with varying concentrations of Stx1 for 2 hours. Nuclear extracts were prepared and electrophoretic mobility shift assays were performed in the presence of a [32P]-labeled double-stranded NF-κB-binding oligonucleotide or an oligonucleotide containing a substitution in the NF-κB binding motif. The data shown in (C) are the ratios of counts in Stx1-treated cells per counts in control cells (basal) ± SEM from three separate experiments.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/92/2/10.1182_blood.v92.2.558/5/blod41435004bw.jpeg?Expires=1726834496&Signature=2q0jObFcKOXWjwlkaN7L5Jjo~nhcydegACXm1IryxOrbQ6ne-R7sJROO5OlH80AoZmvKyTx3UbErJ0RCj~gURoEjYurUt6Kicwvs5Sion3KzwCHM1jcE4t0xwXhU0fd9dR8ii2O5Wci5Ywo9IhifRX5BMIXIFyCihmeDGgrDjOd1GXdGyPK-uTJ4mI~ZZWQwgRTy92yzcSCbTud2IhAviG134hplSFMevPeeol72nqAu5UNJROX-0h0PTLYTSZlMySp1scH1GQrO5vwEO9dOQfz1QHh10Sh1R7dnMtmV7BtThNCTBeXIXmG7PcibXnKUbzEST6fQCwhhixzDglY3Gg__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Stx1 dose response of NF-κB nuclear translocation. (A) Differentiated THP-1 cells or (B) human PBMn were incubated with varying concentrations of Stx1 for 2 hours. Nuclear extracts were prepared and electrophoretic mobility shift assays were performed in the presence of a [32P]-labeled double-stranded NF-κB-binding oligonucleotide or an oligonucleotide containing a substitution in the NF-κB binding motif. The data shown in (C) are the ratios of counts in Stx1-treated cells per counts in control cells (basal) ± SEM from three separate experiments.
