Fig. 5.
Fig. 5. Induction of SNAP-23 by the stimulation with various cytokines. Factor-dependent cell lines MC9, CTLL-2, and L-G3 were deprived of serum and growth factors, then stimulated with mIL-3, mSCF, mIL-10, mIL-2,or hG-CSF for the indicated time (h). SKT6 cells and M1 cells were stimulated with hEPO and mLIF for the indicated time (h), respectively. Poly(A)+ RNA (1 μg/lane) was separated from the cells and blotted with SNAP-23 and G3PDH probes.

Induction of SNAP-23 by the stimulation with various cytokines. Factor-dependent cell lines MC9, CTLL-2, and L-G3 were deprived of serum and growth factors, then stimulated with mIL-3, mSCF, mIL-10, mIL-2,or hG-CSF for the indicated time (h). SKT6 cells and M1 cells were stimulated with hEPO and mLIF for the indicated time (h), respectively. Poly(A)+ RNA (1 μg/lane) was separated from the cells and blotted with SNAP-23 and G3PDH probes.

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