Fig. 5.
![Fig. 5. Characterization of CSB-induced PBMC proliferation. (A) PBMC were cultured as described in Materials and Methods, and CSB was added at the indicated concentrations. (B) PBMC were treated with CSB (1 mg/mL) in the absence or presence of rTGFβ1 (1 ng/mL). (C) Total PBMC or monocyte-depleted PBMC (nonadherent) were incubated with CSB (1 mg/mL) for 6 days. Monocytes were depleted by adherence to tissue culture flasks and nonadherent cells were collected, counted, and plated. Data shown are [3H]-thymidine incorporation during the final 16 hours of a 6-day culture period. In each case, the results shown are representative of at least three similar experiments.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/92/1/10.1182_blood.v92.1.223.413k15_223_229/5/blod41315005x.jpeg?Expires=1722779349&Signature=wzNru798Z4qYn6K1bhCibQ98rSvleh3BnRWfbDsQU0rmjfVvMtE1yqvyITdwuOyBmS2jeGTtDVLBpu1wPkRG5fpUdvPdhyi5BXeu4rRzG~e4Pa56Bl9Zo3xQ39HtR0b2b3qMcH5gfcaDkheIZGDFWWVn5e~gBQmoThy~dLdxd8mEZZ21OK7PAshMKwHr3pZGDfFYqZ-z2k9h-namQR7Gyffcb-zSexeIHg7fMTZGEXqF8VNzygYCwXGUaZh6urZu41Ei0qByUlEPz3UBrXhDWVoX~65VWRXk4dYA1B0e0z5VNgv0~eDIc~xLWqgzERNOf0cINCqJYvOesc3duYSwuw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Characterization of CSB-induced PBMC proliferation. (A) PBMC were cultured as described in Materials and Methods, and CSB was added at the indicated concentrations. (B) PBMC were treated with CSB (1 mg/mL) in the absence or presence of rTGFβ1 (1 ng/mL). (C) Total PBMC or monocyte-depleted PBMC (nonadherent) were incubated with CSB (1 mg/mL) for 6 days. Monocytes were depleted by adherence to tissue culture flasks and nonadherent cells were collected, counted, and plated. Data shown are [3H]-thymidine incorporation during the final 16 hours of a 6-day culture period. In each case, the results shown are representative of at least three similar experiments.
