The dual differentiation potential of CD11b^+hiCD11c⁺ precursors. (A) Day 6 sorted CD11b^+hiCD11c⁺ DC precursors from Lin⁻c-kit⁺ HPCs were cultured in presence of GM-CSF + TNF-α and M-CSF, respectively, for 5 to 8 additional days. At days 12 to 14, the phenotypes of cells were reanalyzed using double-color immunofluorecence as indicated. For double-color immunostaining, PE-conjugated anti-Ia was used, whereas biotinylated anti-CD11c and CD86 were shown by FITC-conjugated streptavidin and anti–DEC-205 was shown by FITC-labeled anti-rat IgG. The representative expression shown here is one of more than five experiments. (B) A schematic DC differentiation model in vitro from Lin⁻c-kit⁺ HPCs.