The day 6 DC precursors differentiate into cells displaying a dendritic cell morphology at days 10 to 14. CD11b^−/dullCD11c⁺ and CD11b^+hiCD11c⁺ precursors were sorted from murine Lin⁻c-kit⁺ HPC cultures stimulated with GM-CSF + SCF + TNF-α for 6 days. A phase contrast microscopical observation (A, D, G, and H) and May-Grünwald-Giemsa staining (B, C, E, F, and I) were performed on sorted CD11b^−/dullCD11c⁺ and CD11b^+hiCD11c⁺ precursors or after culture for 5 to 8 additional days in the presence of GMCSF + TNF-α or M-CSF, respectively. (A and C) CD11b^−/dullCD11c⁺ precursors cultured for 5 to 6 days in the presence of GM-CSF + TNF-α; (B) sorted CD11b^−/dullCD11c⁺ precursors; (D and F) CD11b^+hiCD11c⁺precursors cultured for additional 5 to 8 days in presence of GM-CSF + TNF-α; (E) sorted CD11b^+hiCD11c⁺ precursors; (G) CD11b^−/dullCD11c⁺ precursors cultured in the presence of M-CSF for 3 days; (H and I) cultured CD11b^+hiCD11c⁺ precursors in the presence of M-CSF for 5 to 8 days. Original magnifications: (A) ×200; (D, G, and H) ×160; (B, C, E, F, and I) ×400.