Fig. 6.
Fig. 6. Western blot analysis of PSer133-CREB and total CREB protein, performed in HEL cells stimulated with 100 ng/mL TPO (lanes 1 through 4), 10−5 mol/L FK (lanes 5 and 6), 10−7 mol/L PMA (lanes 7 through 10), or left untreated (lane 11) in the absence (lanes 1, 5, 7, and 9) or presence of 10−6 mol/L BIM (lanes 2 and 8), 10−4 mol/L chelerythrine (lanes 3 and 10), or 10−6 mol/L H89 (lane 6). Results from one representative experiment of three separate experiments are shown. The densitometric analysis is expressed in arbitrary units (a.u.).

Western blot analysis of PSer133-CREB and total CREB protein, performed in HEL cells stimulated with 100 ng/mL TPO (lanes 1 through 4), 10−5 mol/L FK (lanes 5 and 6), 10−7 mol/L PMA (lanes 7 through 10), or left untreated (lane 11) in the absence (lanes 1, 5, 7, and 9) or presence of 10−6 mol/L BIM (lanes 2 and 8), 10−4 mol/L chelerythrine (lanes 3 and 10), or 10−6 mol/L H89 (lane 6). Results from one representative experiment of three separate experiments are shown. The densitometric analysis is expressed in arbitrary units (a.u.).

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