Fig. 2.
![Fig. 2. Conversion of 5-FC into 5-FU in PBSCs, MCF-7 BCCs, and an 0.1% mixture of MCF-7 cells in PBSCs after exposure to Ad.CMV-CD adenoviral vector. Cells (2.5 × 106) were exposed for 90 minutes to 100 MOI of Ad.CMV-CD and then incubated for 48 hours in a 25-cm2 flask with 10 mL of medium containing 500 μmol/L 5-FC and 40 μCi of [6-3H]5-FC. One milliliter of medium was collected after 24 hours, mixed with an equal amount of ice-cold methanol, and analyzed by HPLC as described in the Materials and Methods. The numbers above each histogram column indicate the total amount of drug in micromoles (either 5-FC or 5-FU) per flask.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/92/2/10.1182_blood.v92.2.672/5/blod41419002x.jpeg?Expires=1726827979&Signature=ls5PZM7x7LzKKWS3sqDJh5lINZJcE1Lubv~3gsHspbWB5c0AuwFUfcgbyXRMKske~DWlg0g7MgvpmIas0JCIwQjrAjmS0EcdkeVq1KHyj85rtuZ-px9G~Y~FWX5DQEFM76ImjhGRLFq5VK1OgDxM8VAgupB7Vl-sTTV415Rrn0SdTZxBEhTCVa3Z5QyNKgkg1nIQjPh18K2Uw-A0~f2zPxKgSKd7~DvIe8ueCTDmQMrtdbhk04oOpY8--QgEJMQOWq4KNcdEqu85MEGDBGX3vheju~gy-W1IBBfdeTmZ2Yz2umWWE~IX-6DyKJ-fCMkUHVTfeU-qnP25WHXlsKr8qg__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Conversion of 5-FC into 5-FU in PBSCs, MCF-7 BCCs, and an 0.1% mixture of MCF-7 cells in PBSCs after exposure to Ad.CMV-CD adenoviral vector. Cells (2.5 × 106) were exposed for 90 minutes to 100 MOI of Ad.CMV-CD and then incubated for 48 hours in a 25-cm2 flask with 10 mL of medium containing 500 μmol/L 5-FC and 40 μCi of [6-3H]5-FC. One milliliter of medium was collected after 24 hours, mixed with an equal amount of ice-cold methanol, and analyzed by HPLC as described in the Materials and Methods. The numbers above each histogram column indicate the total amount of drug in micromoles (either 5-FC or 5-FU) per flask.
