RT-PCR analysis of viral BZLF-1 and EBNA-2 mRNA expression. Neutrophils were incubated in absence (control) or in presence of EBV for various times (15 minutes and 5, 10, and 20 hours). Total RNA was reverse transcribed and amplified using specific BZLF-1 and EBNA-2 primers as described in Materials and Methods. Control cells were the EBV⁺ B95-8 cells line for BZLF-1 analysis and the EBV⁺ Raji cells line for EBNA-2 analysis. Amplification were Southern blotted and probed as described in Materials and Methods. The size of PCR products are 182 bp for BZLF-1 and 381 bp for EBNA-2. Results are representative of three different donors.