American Society of Hematology

Fig. 5.

$Fig. 5. Constitutive IL-10 mRNA and β2-microglobulin expression in control and G-PBMC. (A) RT-PCR for IL-10 and β2-microglobulin was performed from mRNA extracts of unfractionated PBMC or sorted CD4 and CD14 cells as described in Materials and Methods. The purity of sorted populations exceeded 96%. (B) Mean quantitations (ImageQuant software) of duplicate amplification products were normalized against signals obtained for β2-microglobulin. Shown is one of two experiments using paired control and G-PBMC samples from each of two donors.$

Constitutive IL-10 mRNA and β₂-microglobulin expression in control and G-PBMC. (A) RT-PCR for IL-10 and β₂-microglobulin was performed from mRNA extracts of unfractionated PBMC or sorted CD4 and CD14 cells as described in Materials and Methods. The purity of sorted populations exceeded 96%. (B) Mean quantitations (ImageQuant software) of duplicate amplification products were normalized against signals obtained for β₂-microglobulin. Shown is one of two experiments using paired control and G-PBMC samples from each of two donors.

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