Fig. 4.
The effects of anti-Fas antibody (Jo2) treatment on CFU-GM. The mice were injected with 5 × 107 of B6 spleen cells (B6/BDF1) or 1 × 108 of D2 cells (D2/BDF1). On day 14 after transfer, the mice were killed to remove their BM cells. The cells were treated with 1 mg/mL of Jo2 antibody for 18 hours. A CFU-GM assay was performed in methylcellulose culture containing 105 BM cells. The number of colonies was then counted after 14 days of culture. The results from four mice were expressed as the mean ± SEM. (▧), αFas(−); (▧), αFas(+). *Significant decrease versus the cells incubated without anti-Fas antibody (P < .01).
