Analysis of PBMC for evidence of the neo marker and for EBV DNA. (A) Levels of neo DNA are shown in the upper portion. They were measured by semiquantitative PCR analysis of 1 μg total DNA at various time points after the second CTL infusion. Lower portion shows EBV DNA in PBMC for the corresponding time points. Levels of EBNA 2 DNA were measured by nested semiquantitative PCR analysis of 1 μg total DNA. (B) Detection of marker gene in pleural fluid of patient no. 13, 3 weeks after CTL infusion. Detection in the infused CTL line and blood obtained on the same day are shown for comparison.