Multiple PCR analysis of bcl-2/J_H junction region using primers specific for the major translocation breakpoint region (MBR). Total DNA extracts of lymph node from 3 cases of HD (cases a, b, and c) were amplified, separated on 2% agarose/NuSieve gel electrophoresis, transferred to nylon membrane, and hybridized with³²P-endlabeled MBR-P⁺ oligonucleotide probe specific for the bcl-2/MBR region. BL (blank), PCR amplification without DNA template to rule out contamination; C+, positive control [t(14;18)-positive tumor DNA]. Analysis of the different samples shows rearranged bcl-2/J_H bands within the expected range of size (150 to 400 bp). Note the presence of rearranged bands of the same size in five (cases a and b) or four (case c) of the five PCR runs performed on the same DNA sample in each case. Note also that the sizes of these bands vary from one case to another.