Fig. 6.
Fig. 6. NK cells and DCs develop simultaneously through a common CD44bright intermediate stage. Cells derived from CD34+CD33lo thymoctes cultured with IL-7, IL-1α, IL-6, SCF, and GM-CSF were recovered at day 2 and cultured for 5 additional days with the same cytokine mixture either with or without IL-2. (A) Shows CD44 expression versus cell size (FS, forward scatter; left plot) and the correlated expression of CD7 and CD13 on electronically gated CD44bright large (forward scatter >350) cells (right plot) at day 2. (B) Shows the kinetics of CD7 and CD13 expression on electronically gated CD44bright large cells recovered at the time points indicated after reculture with the cytokine mixture either with or without IL-2.

NK cells and DCs develop simultaneously through a common CD44bright intermediate stage. Cells derived from CD34+CD33lo thymoctes cultured with IL-7, IL-1α, IL-6, SCF, and GM-CSF were recovered at day 2 and cultured for 5 additional days with the same cytokine mixture either with or without IL-2. (A) Shows CD44 expression versus cell size (FS, forward scatter; left plot) and the correlated expression of CD7 and CD13 on electronically gated CD44bright large (forward scatter >350) cells (right plot) at day 2. (B) Shows the kinetics of CD7 and CD13 expression on electronically gated CD44bright large cells recovered at the time points indicated after reculture with the cytokine mixture either with or without IL-2.

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