CD34⁺CD33^lo thymocytes develop simultaneously into NK Cells and DCs in multicytokine-supported cultures containing IL-2. CD34⁺ CD33^lothymocytes were cultured with the mixture of IL-7, IL-1α, IL-6, SCF, and GM-CSF described in Fig 2, plus IL-2. (A) Depicts the correlated expression of CD44 versus CD5, CD44 versus CD34, and CD34 versus CD33 at day 4. CD34⁺CD44^brightCD5^lo/− cells represent 90% of total cells. High surface CD33 expression was observed on 75% of total cells. (B) Shows the phenotype of the cellular progeny at day 8. Cells were analyzed for the correlated expression of CD7, CD13, and either CD1, CD4, or CD56. Monoparametric histograms show the expression of CD1, CD4, and CD56 (shaded areas) on the CD7⁺CD13^lo/− and CD7^lo/−CD13⁺ cell subsets electronically gated as shown in the biparametric plot. Background fluorescence (unshaded histograms) was determined by staining with isotype-matched irrelevant MoAbs plus PE-Cy5-conjugated goat antimouse Igs.