Fig. 5.
Growth of XG-1 tumor cells in vitro. Tumors growing in SCID mice grafted with XG-1 cells and injected (A) or not (B) with agonist anti-gp130 MoAbs were put in suspension and viable cells were cultured at a concentration of 2.5 × 105 cells/mL in culture medium supplemented with 10 μg/mL of control murine IgG1 (control), 3 ng/mL of IL-6 (IL-6), or 10 μg/mL of a mixture of the B-S12 and B-P8 anti-gp130 MoAbs (5 μg each; anti-gp130 antibodies). Every 5 days, cells were counted and diluted at the initial cell concentration with fresh culture medium and the initial concentration of cytokine or antibodies. Results are the mean cumulative numbers of cells determined on six different culture wells.
