Fig. 4.
![Fig. 4. Effect of a CXCR-4 blocking antibody on transendothelial migration of CXCR-4–positive primary AML blasts. Migration was expressed as relative transendothelial migration compared with migration in response to MS-5–conditioned medium (CM [MS-5] = 100%). The CXCR-4 antibody (mAb) 12G5 markedly reduced migration of CXCR-4–positive, primary AML blasts (AML no. 7, 8, 11, and 12) in response to SDF-1–containing conditioned medium (CM + mAb). Transendothelial migration induced by 500 ng/mL rhSDF-1β was nearly as efficient as migration induced by the conditioned medium. The chemotactic effect of both MS-5–conditioned medium and recombinant SDF-1 was reduced by the partially blocking CXCR-4 antibody to a similar extent.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/91/12/10.1182_blood.v91.12.4523/4/blod41204004x.jpeg?Expires=1724250517&Signature=Vxpz8A9vaGeybvQrorfXjBxanzwi~Gnauoni3j~g2R3kAzL4GdwbdufGcM3Ke5ghUz490EpkzO405pfDsQo2~G7hzRAoQzZAbqlLmIMMMbiK9yadx7mzuooJyaqIEl9hzIpcbLYdg83xyzDXsumu6sLgN5yrMJC~UnV7Y0a15jiCPUKUizmb--dBRbU32PuQqTq7W6wzPRjrleTJGVYWOW9g9MAbnM-MrjphvnDCxvw8ylmnWKZb5jBsuj~OR2Y6uHzDQqeA~CDAuMEXn5JqKpv06VQzwive26Y0jJWAFD-yMaGIGkjsUOZ37anxtDnyDKhIdwrPZdyHa5G68fiUiw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Effect of a CXCR-4 blocking antibody on transendothelial migration of CXCR-4–positive primary AML blasts. Migration was expressed as relative transendothelial migration compared with migration in response to MS-5–conditioned medium (CM [MS-5] = 100%). The CXCR-4 antibody (mAb) 12G5 markedly reduced migration of CXCR-4–positive, primary AML blasts (AML no. 7, 8, 11, and 12) in response to SDF-1–containing conditioned medium (CM + mAb). Transendothelial migration induced by 500 ng/mL rhSDF-1β was nearly as efficient as migration induced by the conditioned medium. The chemotactic effect of both MS-5–conditioned medium and recombinant SDF-1 was reduced by the partially blocking CXCR-4 antibody to a similar extent.
