Fig. 6.
Fig. 6. Inhibition of Mac-1 and Gr-1 differentiation antigen induction by WT1 expression. Control (V4, V5, and V6) and wild-type W T1-infected (W2, W4, W10, and W12) 32D clones were cultured in IL-3 (100 U/mL)–containing or G-CSF (20 ng/mL)–containing medium for 10 days. The cells were stained with FITC-conjugated M1/70.15 MoAb (CD 11b, Mac-1) or FITC-conjugated RB6-8C5 MoAb (Gr-1), and then FACS-analyzed. Means ± 2 SD of positive cells in three control clones and those in four WT1-infected clones are shown.

Inhibition of Mac-1 and Gr-1 differentiation antigen induction by WT1 expression. Control (V4, V5, and V6) and wild-type W T1-infected (W2, W4, W10, and W12) 32D clones were cultured in IL-3 (100 U/mL)–containing or G-CSF (20 ng/mL)–containing medium for 10 days. The cells were stained with FITC-conjugated M1/70.15 MoAb (CD 11b, Mac-1) or FITC-conjugated RB6-8C5 MoAb (Gr-1), and then FACS-analyzed. Means ± 2 SD of positive cells in three control clones and those in four WT1-infected clones are shown.

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