American Society of Hematology

Fig. 3.

$Fig. 3. Inability of cAMP and C60(D3) to prevent Fas-induced apoptosis as assessed by annexin V binding. Jurkat cells were treated with CH-11 and inhibitors for 14 hours as described in Fig 2. Cells were washed, resuspended in annexin V-FITC (1 μg/mL; Clontech), and analyzed on FACScan. M1 is designated as the fraction of apoptotic cells. In data not shown, cells treated with ceramide were similarly assayed. cAMP, (C60)D3, and ICEi were used at the same concentration as in Fig 2B.$

Inability of cAMP and C60(D3) to prevent Fas-induced apoptosis as assessed by annexin V binding. Jurkat cells were treated with CH-11 and inhibitors for 14 hours as described in Fig 2. Cells were washed, resuspended in annexin V-FITC (1 μg/mL; Clontech), and analyzed on FACScan. M1 is designated as the fraction of apoptotic cells. In data not shown, cells treated with ceramide were similarly assayed. cAMP, (C60)D3, and ICEi were used at the same concentration as in Fig 2B.

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