American Society of Hematology

Fig. 6.

$Fig. 6. (A) RT-PCR analysis of CCR-5 message in thymocytes. Messenger RNA for CCR-5 was detected as described in the Materials and Methods section. An unfractionated population of thymocytes (2 × 106 total cells) was used for the reaction. Freshly isolated thymocytes were analyzed for migration capacity in response to MIP-1β (not shown) then aliquots frozen for molecular analyses. Human CCR-5 cDNA, cloned into the pBluescript Sk + vector (Stratagene), was used as a positive control, for the specificity of the primers outlined in the Methods. (B) Identical experiments were performed with thymocyte subpopulations (8 × 106) with or without the addition of reverse transcriptase enzyme.$

(A) RT-PCR analysis of CCR-5 message in thymocytes. Messenger RNA for CCR-5 was detected as described in the Materials and Methods section. An unfractionated population of thymocytes (2 × 10⁶ total cells) was used for the reaction. Freshly isolated thymocytes were analyzed for migration capacity in response to MIP-1β (not shown) then aliquots frozen for molecular analyses. Human CCR-5 cDNA, cloned into the pBluescript Sk + vector (Stratagene), was used as a positive control, for the specificity of the primers outlined in the Methods. (B) Identical experiments were performed with thymocyte subpopulations (8 × 10⁶) with or without the addition of reverse transcriptase enzyme.

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