Fig. 3.
![Fig. 3. Effect of FVII(a)-immunodepleted ACSET [A(ID)] on VEGF production. Confluent fibroblasts were incubated for 24 hours in the presence of 100 nmol/L FVII(a) or immunodepleted ACSET [A(ID)] used at the same protein concentration as FVII(a). Secreted VEGF was assessed by a specific ELISA. Results are expressed as the fold induction of VEGF production, with VEGF secretion by unstimulated fibroblasts (NS) being considered as onefold induction and corresponding to 109.4 ± 14.6 pg/mL of secreted VEGF. Each point represents the mean ± SEM of at least eight different determinations each performed in triplicate. *P < .01 versus unstimulated and A(ID)-stimulated fibroblasts.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/91/8/10.1182_blood.v91.8.2698.2698_2698_2703/5/blod40855003x.jpeg?Expires=1722776076&Signature=bmgz4fuQZEtlv8ZgIt8r0BcHtx0CUPDI2QzcXQVyBq2qo9YX8xlU8K9N86hK1VgaRbdpIJPQDuvkeR5AYTT656WIfLUV2c4hzbnIfnKoYOVyZTy3KlM4kP9arduG02co2yNPjh36pviWLKRCy0PktfZ17zNBP5FhG7tj7hyVbRjB7EuHG8HSX0k~eCMXjpI8jKk-Mu4CJpZh~HUXonZRzmrr1kMiDdbg7MRJ9GBmAyL3BjKLQFqJwtqTt277xOi6S1fLe6geWa7xP4A1j5Mlxjb61D71~ReXkPeetX5v9Yi89GWO3hYKE6~PULq60BbYr4~FVpM1TvFoE1Ii7B~CxA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Effect of FVII(a)-immunodepleted ACSET [A(ID)] on VEGF production. Confluent fibroblasts were incubated for 24 hours in the presence of 100 nmol/L FVII(a) or immunodepleted ACSET [A(ID)] used at the same protein concentration as FVII(a). Secreted VEGF was assessed by a specific ELISA. Results are expressed as the fold induction of VEGF production, with VEGF secretion by unstimulated fibroblasts (NS) being considered as onefold induction and corresponding to 109.4 ± 14.6 pg/mL of secreted VEGF. Each point represents the mean ± SEM of at least eight different determinations each performed in triplicate. *P < .01 versus unstimulated and A(ID)-stimulated fibroblasts.
