Fig. 1.
Fig. 1. (A) Kinetics of FVII(a)-induced VEGF secretion by human fibroblasts. Confluent fibroblasts were incubated with 100 nmol/L FVII(a) for 24 and 48 hours at 37°C. Secreted VEGF was assessed in a specific ELISA. Results are expressed as the fold induction of VEGF in FVII(a)-treated fibroblasts as compared with unstimulated fibroblasts at the same time. Each point represents the mean ± SEM of four different determinations each performed in triplicate. (B) Concentration effect of FVII(a) on VEGF production. Confluent fibroblasts were incubated for 24 hours with or without 10, 25, 50, 75, or 100 nmol/L of FVII(a) at 37°C. Secreted VEGF was assessed by a specific ELISA. Results are expressed as the fold induction of VEGF secretion as compared with unstimulated fibroblasts (basal VEGF level, 82.29 ± 11.8 pg/mL). Each point represents the mean ± SEM of three different determinations each performed in triplicate. *P < .05 versus unstimulated fibroblasts.

(A) Kinetics of FVII(a)-induced VEGF secretion by human fibroblasts. Confluent fibroblasts were incubated with 100 nmol/L FVII(a) for 24 and 48 hours at 37°C. Secreted VEGF was assessed in a specific ELISA. Results are expressed as the fold induction of VEGF in FVII(a)-treated fibroblasts as compared with unstimulated fibroblasts at the same time. Each point represents the mean ± SEM of four different determinations each performed in triplicate. (B) Concentration effect of FVII(a) on VEGF production. Confluent fibroblasts were incubated for 24 hours with or without 10, 25, 50, 75, or 100 nmol/L of FVII(a) at 37°C. Secreted VEGF was assessed by a specific ELISA. Results are expressed as the fold induction of VEGF secretion as compared with unstimulated fibroblasts (basal VEGF level, 82.29 ± 11.8 pg/mL). Each point represents the mean ± SEM of three different determinations each performed in triplicate. *P < .05 versus unstimulated fibroblasts.

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