Western blot analysis of p27 protein expression in normal lymphocytes and lymphoproliferative disorders. (A) A membrane containing protein extracts from normal resting B lymphocytes (B₀), normal resting T lymphocytes (T₀), PMA + PHA-stimulated T lymphocytes after 12 (T₁₂) and 72 (T₇₂) hours of culture. (B) A membrane containing protein extracts from peripheral blood lymphocytes of the B-CLL patient serving as positive control (C), and peripheral blood lymphocytes of five B-CLL patients (no. 1 through 5), a patient with MCL (no. 6), and two LPL patients (no. 7 and 8). Each lane was loaded with 100 μg of total protein. Blots were exposed to anti-p27 antibody and anti-HSP 72/73 as protein deposit control.