Fig. 9.
![Fig. 9. Removal of TNFα from DC cultures results in reduced levels of antitetanus and primary allogeneic T-cell stimulation in vitro. Purified CD4+ T cells were cultured with DC generated in the presence of TNFα [denoted TNF DC day 7(+)], absence of TNFα (denoted Non-TNF DC), or after the removal of TNFα from the cultures at 2 days [denoted TNF DC day 2(−)] or 4 days [denoted TNF DC day 4(−)]. Proliferative responses were measured on day 6 at a DC to T-cell ratio of 1:20. The SEM of triplicate wells was always less than 15% of the mean. T cells alone = 1,534 ± 186 cpm; T cells + PHA = 30,081 ± 1,979 cpm. The results are representative of two experiments.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/91/12/10.1182_blood.v91.12.4652/4/blod41203009x.jpeg?Expires=1724265420&Signature=a6dpXxUTrgBYR~jpqU05j~Mf4WJ73~S6j4u0CaU2D4gcBCcsANS9r27XaZ4f4wFhgE~b7GqxbXy2rroyaZGBGU-0bn3Xg9fvNQElFGlgWjLQCYIXk9zh8tJq4urYpJOq9r5IIAxhV1lDT~1HzFQOuNWgvNDbQtAOPPpNm9FbiZM4GLeQKg9PvvFN0ukD4RcsD-WwtfmHuUlkR7Jgx85jpI0C6WQ17E9yQfilM0URvS5nvebuT5W-MT3fKctlgC5wHMqCDcsk9Fm-01~la~u~rqVu1dGOmVjObkFha4oyAI-QpWRYHi2Q4U5gzZNJDj5DEdUchJ74KKDck4sG07LE4w__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Removal of TNFα from DC cultures results in reduced levels of antitetanus and primary allogeneic T-cell stimulation in vitro. Purified CD4+ T cells were cultured with DC generated in the presence of TNFα [denoted TNF DC day 7(+)], absence of TNFα (denoted Non-TNF DC), or after the removal of TNFα from the cultures at 2 days [denoted TNF DC day 2(−)] or 4 days [denoted TNF DC day 4(−)]. Proliferative responses were measured on day 6 at a DC to T-cell ratio of 1:20. The SEM of triplicate wells was always less than 15% of the mean. T cells alone = 1,534 ± 186 cpm; T cells + PHA = 30,081 ± 1,979 cpm. The results are representative of two experiments.
