Fig. 8.
![Fig. 8. Removal of TNFα from DC cultures results in reduced levels of CD80, CD86, and CD83 expression. TNFα added to 7-day DC cultures was subsequently removed by washing the cells 2 and 4 days later [denoted as TNF day 2(−) and TNF day 4(−), respectively]. The cultures were then replated in fresh SFM with added GM-CSF and IL-4 for an additional 5 or 3 days, respectively. Comparisons were made with DC cultured without TNFα (denoted Non-TNF DC) and those cultured in TNF for the complete 7-day period [denoted TNF day 7(+)]. FACS analysis of mean fluorescence channel is depicted. The results are representative of two experiments.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/91/12/10.1182_blood.v91.12.4652/4/blod41203008x.jpeg?Expires=1724264564&Signature=OgkOwiwOUkjzJSsgd6E~zwBCzrnAOUOFyLIgqdoLNj~pQRXxmhXqiw4Ime4DsuRVnayctNeT76M6XiG98W5-00s-DQmGWOL-MSDnxEcGB8s9cMDp~BxVN6cbbUSyBxuCv~LUIRZgMLwKPaSC4SlkyWgUesP8pwTf3Gl6O80N9nRQZU~S99gjWdjGhxnB7jRFedtEvjWDL2iW~-P0Z~T-3Ff3J9qrbKawIiBusqiuT3D4IIcSzlr~~pXfKGcmeHWl6XAf5yMKUCK1ag2YdV5nnPMvFqX41d95Y9oZMSpiW6JIkrZt0Uz-168zTT0h7jJoKWfh0CuLZo1oxCy-KbV0hQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Removal of TNFα from DC cultures results in reduced levels of CD80, CD86, and CD83 expression. TNFα added to 7-day DC cultures was subsequently removed by washing the cells 2 and 4 days later [denoted as TNF day 2(−) and TNF day 4(−), respectively]. The cultures were then replated in fresh SFM with added GM-CSF and IL-4 for an additional 5 or 3 days, respectively. Comparisons were made with DC cultured without TNFα (denoted Non-TNF DC) and those cultured in TNF for the complete 7-day period [denoted TNF day 7(+)]. FACS analysis of mean fluorescence channel is depicted. The results are representative of two experiments.
