Surface phenotype of metrizamide gradient-separated DC detected by a panel of antihuman FITC- or PE-labeled antibodies and FACS analysis. DC were generated from PBMC cultured for 14 days in XVIVO-15 serum-free medium containing GM-CSF and IL-4. The x-axis is a logarithmic scale of fluorescence intensity and the y-axis represents counts. DC showed high-level expression of MHC class II (HLA-DR), CD86, and CD11c molecules.