American Society of Hematology

Fig. 2.

$Fig. 2. Expression of c-maf mRNA in myeloma cell lines and some normal human tissues. (A) A Northern blot containing 15 μg of total RNA from each of 14 MM lines was probed for c-maf, with the horizontal lines indicating the position of 5.0- and 2.0-kb ribosomal RNAs. Ethidium bromide staining is shown in the lower panel. (B) A Northern blot containing 2 μg of poly (A)+ RNA from each of several normal tissues was assessed for c-maf expression. (C) Genomic DNA, cDNA, and RNA from 2 MM cell lines was subjected to PCR amplification using appropriate oligonucleotides from the 3′ untranslated region of c-maf. The amplified products were digested with Mnl I and fractionated by electrophoresis on a 2% acrylamide gel. The positions of Mnl I sites, including the polymorphic (*) Mnl I site, in the amplified fragments are shown.$

Expression of c-maf mRNA in myeloma cell lines and some normal human tissues. (A) A Northern blot containing 15 μg of total RNA from each of 14 MM lines was probed for c-maf, with the horizontal lines indicating the position of 5.0- and 2.0-kb ribosomal RNAs. Ethidium bromide staining is shown in the lower panel. (B) A Northern blot containing 2 μg of poly (A)+ RNA from each of several normal tissues was assessed for c-maf expression. (C) Genomic DNA, cDNA, and RNA from 2 MM cell lines was subjected to PCR amplification using appropriate oligonucleotides from the 3′ untranslated region of c-maf. The amplified products were digested with Mnl I and fractionated by electrophoresis on a 2% acrylamide gel. The positions of Mnl I sites, including the polymorphic (*) Mnl I site, in the amplified fragments are shown.

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