Fig. 3.
Western blot to detect the formation of circulating antibody in the plasma of mice injected with 1 to 2 × 1011 particles of AAV-CMV-F.IX at day 1 of life. Lanes 1 through 8 show plasma samples from eight different animals taken at day 240 postinjection. Samples were diluted 1:500. Identical results were obtained from 1:200 dilutions (data not shown). Animals in lanes 5 and 6 had developed detectable levels of anti-hF.IX.
