Fig. 9.
![Fig. 9. Effect of MPA on CDK6 kinase activity. CDK6-associated kinase activity using affinity-purified antisera against CDK6 and a GST-Rb fusion protein as substrate. Cells were stimulated with IL-2/PHA-L for 24 and 48 hours in absence (lanes 1 to 6) or in presence of 1μmol/L MPA (lanes 7 to 10). Immunoprecipitations were performed with or without incubation with the competing peptide for CDK6. The immune complexes were incubated in kinase buffer containing [γ-32P]ATP and separated by SDS-PAGE and exposed to radiograph film, as outlined in the Materials and Methods section.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/91/8/10.1182_blood.v91.8.2896.2896_2896_2904/5/blod4082109.jpeg?Expires=1722413462&Signature=kywEHT7BYZDfLYN36UZSgBdafO3OPzt1oWhjBU159G-BqSGaOVKisjjyLpb9Vd5R9Mjkkz4W-p~nmifu~kOsJrZi~nhTuOprTYHnl3Q4rIx3hamvhI7OzKU8FNF62L1AFRx2A8Ejf74AKOxFDD3QfXboCIxjj7RZFtFnKEumhKrhRuFhmNfhWy-TjSTNSCWsleG8koaZHh6ZKuvzSareR-L9JFGTJOzmYNoYvM8u~D3zAiskx2gaJMGiv4nDBh-2H0yzIXfZwhg3v~XzaSwwIOgZzIPtWff7LHwlq6-BfUxWENqxotzuS2Oe8MTlLm4TtmgK3HbIkkEMp4Xg0ySqYw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Effect of MPA on CDK6 kinase activity. CDK6-associated kinase activity using affinity-purified antisera against CDK6 and a GST-Rb fusion protein as substrate. Cells were stimulated with IL-2/PHA-L for 24 and 48 hours in absence (lanes 1 to 6) or in presence of 1μmol/L MPA (lanes 7 to 10). Immunoprecipitations were performed with or without incubation with the competing peptide for CDK6. The immune complexes were incubated in kinase buffer containing [γ-32P]ATP and separated by SDS-PAGE and exposed to radiograph film, as outlined in the Materials and Methods section.
