Effect of MPA on CDK6 complex formation. (A) [³⁵S]methionine-labeled lysates of stimulated T lymphocytes were immunoprecipitated with anti-CDK6 antibody (stimulation for 42 hours) with or without preincubation with the competing peptide. Cells were stimulated with IL-2/PHA-L in absence (lanes 1 to 2) or presence of 1 μmol/L MPA (lanes 3 to 4) and 50 μmol/L guanosine and 100 μmol/L 8-aminoguanosine (lanes 5 to 6). (B) Western blot analysis of lysates immunoprecipitated with CDK6 antibody and detected with cyclin D3 antibody. Resting T lymphocytes (lane 1) were stimulated with IL-2/PHA-L for 24 and 48 hours in absence (lanes 2 to 3) or presence of 1 μmol/L MPA (lanes 4 to 5) and 50 μmol/L guanosine and 100 μmol/L 8-aminoguanosine (lanes 6 to 7). The mobility of protein molecular weight standards and relevant proteins are indicated.